Canine PD-1/PDCD1 Protein
Catalog No: PD1-DP001
- Species
- Canine
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Canine PD-1/PDCD1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu25-Val170 (accession: NP_001301026.1).
Product Details
- Molecular Aliases
- PDCD1; PD1; CD279; SLEB2; PD-1
- Protein Length
- Leu25-Val170
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 43.00 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Canine PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine PD-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Canine PD-1, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 47.0ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Canine PD-L1, mFc Avi tag (Cat. PDL-CM610B) at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Canine PD-1, hFc Tag with the EC50 of 53.3ng/ml determined by ELISA.
Canine PD-1, hFc Tag captured on CM5 Chip via ProtienA can bind Human PD-L1, His Tag with an affinity constant of 0.696 μM as determined in SPR assay (Biacore T200).
Background
Programmed cell death protein 1, also known as PD-1 and CD279, is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
References
- Blank C, Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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