Canine HE4 Protein
Catalog No: HE4-DF001
- Species
- Canine
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Canine HE4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly28-Phe124 (UniProt accession: Q28894).
Product Details
- Molecular Aliases
- Epididymal secretory protein E4; CE4; WFDC2; WAP5; EDDM4
- Protein Length
- Gly28-Phe124
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 37.06 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Canine HE4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine HE4 is greater than 95% as determined by SEC-HPLC.
Background
The ovarian cancer biomarker HE4 is known to promote proliferation, metastasis, chemoresistance, and suppression of cytotoxic lymphocytes. HE4 has the ability to affect signaling events and gene expression in multiple cell types of the tumor microenvironment, which could contribute to angiogenesis and altered immunogenic responses in ovarian cancer.
References
- James NE, Emerson JB, Borgstadt AD, Beffa L, Oliver MT, Hovanesian V, Urh A, Singh RK, Rowswell-Turner R, DiSilvestro PA, Ou J, Moore RG, Ribeiro JR. The biomarker HE4 (WFDC2) promotes a pro-angiogenic and immunosuppressive tumor microenvironment via regulation of STAT3 target genes. Sci Rep. 2020 May 22;10(1):8558. doi: 10.1038/s41598-020-65353-x. PMID: 32444701; PMCID: PMC7244765.
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