Biotinylated Rat MASP2 Protein (Primary Amine Labeling)
Catalog No: MASP2-RB001
- Species
- Rat
- Expression System
- E.coli
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Rat MASP2 Protein (Primary Amine Labeling) is expressed in E. coli with a His tag at the C-terminus. It contains amino acid residues Thr287-Phe685 (accession: A2VCV7).
Product Details
- Molecular Aliases
- MAP19; MASP-2; MASP1P1; sMap;MASP2
- Protein Length
- Thr287-Phe685
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in 50mM Tris, 200mM NaCl (pH 9.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Rat MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Narsoplimab, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat MASP2, His Tag with the EC50 of 19.3ng/ml determined by ELISA. (QC Test)
Background
Te MASP2 gene is located on the chromosome 1p36.23–31 and encodes two proteins, MASP-2 and Map198. Both the levels and function of MASP-2 are modulated by the single-nucleotide polymorphisms (SNPs) of the MASP2 gene. Te level of MASP-2 in plasma plays a dual role in the infection and progression of infectious diseases. Te defciency of MASP-2 in plasma has been associated with the susceptibility of HIV. By contrast, the overproduction of MASP-2 may increase the infammatory efect.
References
- Li Z, Wang M, Zhong H, Huang X, Wu X, Zhang X, Wang J, Deng J, Chen M, Chen L, Tan H. Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis. Sci Rep. 2021 Mar 22;11(1):6544. doi: 10.1038/s41598-021-86129-x. PMID: 33753877; PMCID: PMC7985323.
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