Biotinylated Rat GDF15 Protein (Primary Amine Labeling)
Catalog No: GDF15-RB001
- Species
- Rat
- Expression System
- E.coli
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Rat GDF15 Protein (Primary Amine Labeling) is expressed in E. coli with a His tag at the N-terminus. It contains amino acid residues Ser189-Ala303 (UniProt accession: Q9Z0J6).
Product Details
- Molecular Aliases
- GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β; NAG1; RG1; Placental TGF-b; PTGF-b; PTGFBPTGF-b; Growth differentiation factor-15; Growth differentiation factor 15
- Protein Length
- Ser189-Ala303
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 13.68 kDa. The protein migrates to 14-17 kDa under reduced (R) condition and 25-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 50mM HAc.
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Rat GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA.
Background
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
References
- Baek SJ, Eling T. Growth differentiation factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.
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