Biotinylated Mouse PTK7/CCK4 Protein
Catalog No: PTK7-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Mouse PTK7/CCK4 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala23-Thr696 (UniProt accession: Q8BKG3).
Product Details
- Molecular Aliases
- CCK-4; PTK7; CCK4
- Protein Length
- Ala23-Thr696
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 77.48 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse PTK7 is greater than 95% as determined by SEC-HPLC.
Background
Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling.
References
- Lichtig H, Cohen Y, Bin-Nun N, Golubkov V, Frank D. PTK7 proteolytic fragment proteins function during early Xenopus development. Dev Biol. 2019 Sep 1;453(1):48-55. doi: 10.1016/j.ydbio.2019.05.007. Epub 2019 May 21. PMID: 31125531.
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