Biotinylated Mouse IL-21 Protein
Catalog No: IL21-MB001
- Species
- Mouse
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse IL-21 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues His18-Ser146 (UniProt accession: Q9ES17).
Product Details
- Molecular Aliases
- Interleukin-21; IL-21; Za11; CVID11;IL21;IL 21; Interleukin 21
- Protein Length
- His18-Ser146
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.9 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 20mM NaAc (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-21R, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
Background
Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
References
- Sim GC, Radvanyi L. The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth Factor Rev. 2014 Aug;25(4):377-90. doi: 10.1016/j.cytogfr.2014.07.018. Epub 2014 Aug 1. PMID: 25200249.
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