Biotinylated Human ULBP-1 Protein
Catalog No: ULBP1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ULBP-1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly26-Pro215 (UniProt accession: Q9BZM6).
Product Details
- Molecular Aliases
- ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1;ALCAN-β;N2DL1; MULT1;ALCAN-b
- Protein Length
- Gly26-Pro215
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14μg/ml determined by ELISA.
Background
Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.
References
- Schrama D, Terheyden P, Otto K, et al. Expression of the NKG2D ligand UL16 binding protein-1 (ULBP-1) on dendritic cells. Eur J Immunol. 2006;36(1):65-72. doi:10.1002/eji.200535115
Have questions about this product?
Our technical team can help with product selection, application questions and order support.