Biotinylated Human TSLP (R127A, R130A) Protein
Catalog No: TSLP-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Tyr29-Gln159(R127A,R130A) (UniProt accession: Q969D9-1).
Product Details
- Molecular Aliases
- Thymic stromal lymphopoietin; TSLP
- Protein Length
- Tyr29-Gln159
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. (QC Test)
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A), His Tag with the EC50 of 3.2ng/ml determined by ELISA.
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi tag with an affinity constant of 4.87 nM as determined in BLI assay.
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi tag with an affinity constant of 0.22 nM as determined in BLI assay.
Background
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
References
- Corren J, Parnes J R, Wang L, et al. Tezepelumab in Adults with Uncontrolled Asthma[J]. New England Journal of Medicine, 2017, 377(10):936-946.
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