Biotinylated Human Transferrin Protein
Catalog No: TRANSFERRIN-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Transferrin Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Val20-Pro698 (accession: AAH59367).
Product Details
- Molecular Aliases
- Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1;HEL-S71p
- Protein Length
- Val20-Pro698
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 78.11 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin is greater than 95% as determined by SEC-HPLC.
Immobilized Human Transferrin R, His Tag (Cat. TFR-HM102) at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi tag with the EC50 of 14.3ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Human Transferrin, His-Avi tag on Streptavidin-Biosensor can bind Human Transferrin R, His Tag (Cat. TFR-HM102) with an affinity constant of 10.30 nM as determined in BLI assay.
Background
Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
References
- Murakami Y, Saito K, Ito H, Hashimoto Y. Transferrin isoforms in cerebrospinal fluid and their relation to neurological diseases. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(5):198-210. doi: 10.2183/pjab.95.015. PMID: 31080188; PMCID: PMC6742728.
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