Biotinylated Human TPBG/5T4 Protein
Catalog No: TPBG-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human TPBG/5T4 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser32-Ser355 (UniProt accession: Q13641-1).
Product Details
- Molecular Aliases
- M6P1; 5T4AG; WAIF1; TPBG; 5T4
- Protein Length
- Ser32-Ser355
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TPBG is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human TPBG, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).
Background
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
References
- Harrop R, O'Neill E, Stern PL. Cancer stem cell mobilization and therapeutic targeting of the 5T4 oncofetal antigen. Ther Adv Vaccines Immunother. 2019 Jan 25;7:2515135518821623. doi: 10.1177/2515135518821623. PMID: 30719508; PMCID: PMC6348545. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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