Biotinylated Human Semaphorin 4A/SEMA4A Protein
Catalog No: SEMA4A-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Semaphorin 4A/SEMA4A Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly33-His683 (UniProt accession: Q9H3S1-1).
Product Details
- Molecular Aliases
- Semaphorin-4A; Semaphorin-B; SEMA4A
- Protein Length
- Gly33-His683
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 75.31 kDa. Due to glycosylation, the protein migrates to 85-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human Semaphorin 4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Semaphorin 4A is greater than 95% as determined by SEC-HPLC.
Human LILRB2, hFc Tag (Cat. LIL-HM2B2) captured on CM5 Chip via Protein A can bind Biotinylated Human Semaphorin 4A, His Tag with an affinity constant of 34.05 nM as determined in SPR assay (Biacore T200).
Background
Neuroimmune semaphorin 4A (Sema4A), a member of semaphorin family of transmembrane and secreted proteins, is an important regulator of neuronal and immune functions. In the nervous system, Sema4A primarily regulates the functional activity of neurons serving as an axon guidance molecule. In the immune system, Sema4A regulates immune cell activation and function, instructing a fine tuning of the immune response.
References
- Iyer AS, Chapoval SP. Neuroimmune Semaphorin 4A in Cancer Angiogenesis and Inflammation: A Promoter or a Suppressor? Int J Mol Sci. 2018 Dec 30;20(1):124. doi: 10.3390/ijms20010124. PMID: 30598022; PMCID: PMC6337608.
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