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Biotinylated Human Peptide Ready HLA-A*68:01&B2M Monomer Protein

Catalog No: HLAA6801-HB001

Species
Human
Expression System
HEK293
Tag
His, Avi

Product overview

Recombinant Biotinylated Human Peptide Ready HLA-A*68:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*68:01) and Ile21-Met119 (B2M) (accession: CAD7112706.1(HLA-A*68:01) & P61769(B2M)).

Product Details

Molecular Aliases
MHC; HLA-A*68:01; Peptide Ready
Protein Length
Gly25-Thr305
Expression System
HEK293
Theoretical Molecular Weight
The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Supplied as 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4).
State
Liquid
Storage Conditions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data Display

Bis-Tris PAGE
HLAA6801-HB001 Bis-Tris-PAGE-white result

Biotinylated Human Peptide Ready HLA-A*68:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC
HLAA6801-HB001 SEC-HPLC result

The purity of Biotinylated Human Peptide Ready HLA-A*68:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.

Background

HLA-A*68:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*68:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

References

  1. [1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
  2. [2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.

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