Biotinylated Human PDGF R beta/CD140b Protein
Catalog No: PDGFRB-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human PDGF R beta/CD140b Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu33-Phe530 (UniProt accession: P09619).
Product Details
- Molecular Aliases
- JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-β; PDGF R β; PDGFR2; PDGFR-b; PDGFR-beta; PDGF R b
- Protein Length
- Leu33-Phe530
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human PDGF R beta on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human PDGF R beta is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human PDGF R beta, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).
Background
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
References
- PDGFR blockade is a rational and effective therapy for NPM-ALK–driven lymphomas[J]. Nature Medicine, 2012, 18(11):1699-1704.
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