Biotinylated Human PDGF R alpha/PDGFRA Protein
Catalog No: PDGFRA-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln24-Glu524 (UniProt accession: P16234).
Product Details
- Molecular Aliases
- PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2
- Protein Length
- Gln24-Glu524
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human PDGF R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human PDGF R alpha, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).
Background
Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
References
- Shi Y, He G, Lee WC, McKenzie JA, Silva MJ, Long F. Gli1 identifies osteogenic progenitors for bone formation and fracture repair. Nat Commun. 2017;8(1):2043. Published 2017 Dec 11. doi:10.1038/s41467-017-02171-2
Have questions about this product?
Our technical team can help with product selection, application questions and order support.