Biotinylated Human P-Selectin/CD62P Protein
Catalog No: CD62P-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human P-Selectin/CD62P Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Trp42-Ala771 (UniProt accession: P16109).
Product Details
- Molecular Aliases
- FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3
- Protein Length
- Trp42-Ala771
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% saccharose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human P-Selectin, His-Avi tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Background
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
References
- Ataga K I, Kutlar A, Kanter J, et al. Crizanlizumab for the Prevention of Pain Crises in Sickle Cell Disease[J]. New England Journal of Medicine, 2016, 376(5):429-439.
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