Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling)
Catalog No: NRG1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ser2-Lys246 (UniProt accession: Q02297-6).
Product Details
- Molecular Aliases
- Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A
- Protein Length
- Ser2-Lys246
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Her4, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65μg/ml determined by ELISA.
Background
Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.
References
- Liew H, Kim YM, Choi HS, Jang AR, Churchill D, Lee SH, Suh YH. Soluble Neuregulin-1 from Microglia Enhances Amyloid Beta-induced Neuronal Death. CNS Neurol Disord Drug Targets. 2016;15(8):918-926. doi: 10.2174/1871527315666160815160505. PMID: 27577739.
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