Biotinylated Human Nectin-2/CD112 Protein
Catalog No: NECTIN2-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Nectin-2/CD112 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln32-Leu360 (UniProt accession: Q92692-2).
Product Details
- Molecular Aliases
- CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
- Protein Length
- Gln32-Leu360
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Nectin-2, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA (QC Test).
Immobilized BBiotinylated Human Nectin-2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human PVRIG, mFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
Background
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
References
- Bekes I, Löb S, Holzheu I, Janni W, Baumann L, Wöckel A, Wulff C. Nectin-2 in ovarian cancer: How is it expressed and what might be its functional role? Cancer Sci. 2019 Jun;110(6):1872-1882. doi: 10.1111/cas.13992. Epub 2019 May
- PMID: 30843637; PMCID: PMC6549928. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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