Biotinylated Human Nectin-1/PVRL1/CD111 Protein
Catalog No: PVRL1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln31-Thr334 (UniProt accession: Q15223-1).
Product Details
- Molecular Aliases
- CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR; SK12
- Protein Length
- Gln31-Thr334
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Nectin-1 is greater than 90% as determined by SEC-HPLC.
Immobilized Human Nectin-3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Nectin-1, His Tag with the EC50 of 0.52μg/ml determined by ELISA (QC Test).
Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Background
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
References
- Yu Z, Adusumilli P S, Eisenberg D P, et al. Nectin-1 Expression by Squamous Cell Carcinoma is a Predictor of Herpes Oncolytic Sensitivity[J]. Molecular Therapy, 2007, 15(1):103-113.
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