Biotinylated Human NCAM-1/CD56 Protein
Catalog No: NCAM1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NCAM-1/CD56 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu20-Gly718 (UniProt accession: P13591-2).
Product Details
- Molecular Aliases
- N-CAM-1; NCAM-1; NCAM1; CD56; NCAM; MSK39; N-CAM1
- Protein Length
- Leu20-Gly718
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 80.29 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NCAM-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human NCAM-1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Background
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
References
- Guven A, Gray K, Peng KW, Klotz A, Kellogg MD, Narain NR, Kiebish MA. Development and validation of an immunoassay for quantification of NCAM-1 in human plasma. J Pharm Biomed Anal. 2021 Apr 15;197:113981. doi: 10.1016/j.jpba.2021.113981. Epub 2021 Feb 16. PMID: 33657526.
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