Biotinylated Human NaPi2b/SLC34A2 Nanodisc
Catalog No: SLC34A2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NaPi2b/SLC34A2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Leu689 (UniProt accession: O95436-2).
Product Details
- Molecular Aliases
- NaPi-2b; NaPi 2b; NAPI-IIb; NPTIIb; NaPi2b; PULAM;
- Protein Length
- Met1-Leu689
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 88.6 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human NaPi2b Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Lifastuzumab, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human NaPi2b Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-SLC34A2 Antibody 2, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
Biotinylated Human NaPi2b Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Lifastuzumab, hFc Tag with an affinity constant of 2.64 nM as determined in SPR assay (Biacore T200).
Background
SLC34A2, a member of the SLC34 family, is a multipass membrane protein and encodes a type 2b sodium-dependent phosphate transporter, NaPiIIb. It is known that SLC34A2 can mediate transporting inorganic phosphate into epithelial cells via sodium ion cotransport. SLC34A2 also strongly inhibited tumor growth and metastasis ability in an A549 subcutaneous tumor model and lung metastasis mode.
References
- Li Y, Chen X, Lu H. Knockdown of SLC34A2 Inhibits Hepatocellular Carcinoma Cell Proliferation and Invasion. Oncol Res. 2016 Oct 27;24(6):511-519. doi: 10.3727/096504016X14719078133483. PMID: 28281971; PMCID: PMC7838622.
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