Biotinylated Human MSLN/Mesothelin Protein
Catalog No: MSLN-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Glu296-Gly580 (UniProt accession: Q13421-2).
Product Details
- Molecular Aliases
- MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
- Protein Length
- Glu296-Gly580
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.
Immobilized Human CA125, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test).
Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.
Use Biotinylated Human MSLN-hFc-Avi protein to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.
Background
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
References
- Adusumilli P S, Cherkassky L, Villena-Vargas J, et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.
- Morello A, Sadelain M, Adusumilli P S. Mesothelin-Targeted CARs: Driving T cells to Solid Tumors[J]. Cancer Discovery, 2015, 6(2).
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