Biotinylated Human/Mouse/Goat Cpn10/HSPE1 Protein
Catalog No: HSPE1-HB001
- Species
- Human/Mouse/Goat
- Expression System
- E.coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human/Mouse/Goat Cpn10/HSPE1 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Ala2-Asp102 (accession: XP_005676362).
Product Details
- Molecular Aliases
- Cpn10; HSPE1; Hsp10; Chaperonin 10; CPN10; EPF; GROES
- Protein Length
- Ala2-Asp102
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 13.70 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human/Mouse/Goat Cpn10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Cpn10/HSPE, a 10-kDa heat shock protein, is a novel interacting partner of NPAT. A pool of Cpn10 is colocalized with NPAT foci during G1 and S phases in nuclei. Cpn10 is important for S phase progression and cell proliferation. Interaction of Heat Shock Protein Cpn10 with the Cyclin E/Cdk2 Substrate Nuclear Protein Ataxia-Telangiectasia (NPAT) Is Involved in Regulating Histone Transcription
References
- Ling Zheng L, Wang FY, Cong XX, Shen Y, Rao XS, Huang DS, Fan W, Yi P, Wang XB, Zheng L, Zhou YT, Luo Y. Interaction of Heat Shock Protein Cpn10 with the Cyclin E/Cdk2 Substrate Nuclear Protein Ataxia-Telangiectasia (NPAT) Is Involved in Regulating Histone Transcription. J Biol Chem. 2015 Dec 4;290(49):29290-300. doi: 10.1074/jbc. M115.659201. Epub 2015 Oct 1. PMID: 26429916; PMCID: PMC4705935.
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