Biotinylated Human Midkine Protein
Catalog No: MIDKINE-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human Midkine Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Val21-Asp143 (UniProt accession: P21741-1).
Product Details
- Molecular Aliases
- MK; ARAP; MDK; MEK; MK1; MKARAP; NEGF2
- Protein Length
- Val21-Asp143
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 16.46 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Midkine on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human malignancies and acts as a mediator for the acquisition of critical hallmarks of cancer, including cell growth, survival, metastasis, migration, and angiogenesis.
References
- Filippou PS, Karagiannis GS, Constantinidou A. Midkine (MDK) growth factor: a key player in cancer progression and a promising therapeutic target. Oncogene. 2020 Mar;39(10):2040-2054. doi: 10.1038/s41388-019-1124-8. Epub 2019 Dec 4. PMID: 31801970.
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