Biotinylated Human Mature TGF beta 3 Protein
Catalog No: TGFB3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Mature TGF beta 3 Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Ala301-Ser412 (UniProt accession: P10600-1).
Product Details
- Molecular Aliases
- TGF-beta-3; LAP; TGFB3; ARVD; ARVD1; RNHF;TGF-β-3; TGF-beta 3;TGF-b-3; TGF-b3
- Protein Length
- Ala301-Ser412
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14.53 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Mature TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human TGF-beta RII, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test).
Background
Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.
References
- Kuşcu NK, Laçin S, Vatansever S, Yildirim Y, Var A, Uyanik BS, Koyuncu F. Immunolocalization of transforming growth factor-beta 3 in pregnant human myometrium. Acta Obstet Gynecol Scand. 2001 Dec;80(12):1079-83. PMID: 11846702.
- Pelton RW, Dickinson ME, Moses HL, Hogan BL. In situ hybridization analysis of TGF beta 3 RNA expression during mouse development: comparative studies with TGF beta 1 and beta 2. Development. 1990 Oct;110(2):609-20. doi: 10.1242/dev.110.2.609. PMID: 1723948.
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