Biotinylated Human Mature TGF beta 2 Protein
Catalog No: TGFB2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Ala303-Ser414 (UniProt accession: P61812-1).
Product Details
- Molecular Aliases
- TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2;TGF beta R2; TbR-II; T beta R-II; TGF-b 2
- Protein Length
- Ala303-Ser414
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14.53 kDa same as Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Biotinylated Human Mature TGF beta 2, Avi tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).
Background
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
References
- Yao K, Tan J, Gu WZ, Ye PP, Wang KJ. Reactive oxygen species mediates the apoptosis induced by transforming growth factor beta(2) in human lens epithelial cells. Biochem Biophys Res Commun. 2007 Mar 2;354(1):278-83. doi: 10.1016/j.bbrc.2006.12.198. Epub 2007 Jan 10. PMID: 17217916.
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