Biotinylated Human LPAR1 Nanodisc
Catalog No: LPAR1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human LPAR1 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val364 (UniProt accession: Q92633-1).
Product Details
- Molecular Aliases
- LPA receptor 1; LPA-1; EDG2; LPA1; Gpcr26
- Protein Length
- Met1-Val364
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 54.10 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. (QC Test)
Biotinylated Human LPAR1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-LPAR1 Antibody, hFc Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).
Background
Lysophosphatidic acid receptor 1 (LPA1) belongs to the G protein-coupled receptor family. The ligand for LPA1 is LPA, the simplest lysophospholipid. The mature form of LPA1 is glycosylated and localized on the plasma membrane. LPA1 is bound to heterotrimetric G proteins and transduces intracellular signaling in response to ligation to LPA. Desensitization of LPA1 negatively regulates LPA1-mediated signaling and the resulting biological functions.
References
- Zhao J, Stephens T, Zhao Y. Molecular Regulation of Lysophosphatidic Acid Receptor 1 Maturation and Desensitization. Cell Biochem Biophys. 2021 Sep;79(3):477-483. doi: 10.1007/s12013-021-00999-6. Epub 2021 May 25. PMID: 34032994; PMCID: PMC8887818.
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