Biotinylated Human LILRB3/CD85a/ILT5 Protein
Catalog No: LILRB3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly24-Glu443 (UniProt accession: O75022-1).
Product Details
- Molecular Aliases
- CD85a; ILT5; ILT-5; LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; PIRB; HL9; LIR3MGC138403
- Protein Length
- Gly24-Glu443
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human LILRB3, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Background
Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
References
- Huang J, Burke P S, Cung T D H, et al. Leukocyte Immunoglobulin-Like Receptors Maintain Unique Antigen-Presenting Properties of Circulating Myeloid Dendritic Cells in HIV-1-Infected Elite Controllers[J]. Journal of Virology, 2010, 84(18):9463-9471.
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