Biotinylated Human Latent TGF beta 1/TGFB1 Protein
Catalog No: TGFB1-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Leu30-Ser390(C33S) (UniProt accession: P01137).
Product Details
- Molecular Aliases
- CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1; TGF-beta 1;TGF b1; TGF beta 1; TGF beta; TGF-b-1; TGF-b1
- Protein Length
- Leu30-Ser390
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa (LAP) / 13-15 kDa (Mature TGF beta 1) based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Latent TGF beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Human Latent TGF beta 1 is greater than 85% as determined by SEC-HPLC.
Immobilized Human ITGAV&ITGB6, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 62.5ng/ml determined by ELISA (QC Test).
Immobilized Human ITGAV&ITGB8, His Tag (Cat. ITG-HM4V8) at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Avi tag with the EC50 of 27.2ng/ml determined by ELISA.
Background
Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
References
- Robertson I B, Rifkin D B. Regulation of the Bioavailability of TGF-β and TGF-β-Related Proteins[J]. Cold Spring Harbor Perspectives in Biology, 2016, 8(6):a021907.
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