Biotinylated Human LAIR2/CD306 Protein
Catalog No: LAIR2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human LAIR2/CD306 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln22-Pro152 (UniProt accession: Q6ISS4-1).
Product Details
- Molecular Aliases
- LAIR-2; CD306; LAIR2; MGC71634; XXbac-BCX535A19.6
- Protein Length
- Gln22-Pro152
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Huma LAIR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Huma LAIR2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human LAIR2, His-Avi tag immobilized on CM5 Chip can bind Native Human Collagen I, No Tag with an affinity constant of 1.80 nM as determined in SPR assay (Biacore T200).
Background
The leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) shows increased expression during pregnancy and its reduced expression is associated with pregnancy complications. Although LAIR2 was expressed in the human placenta villus and decidua in the first trimester of pregnancy, it was not expressed in mouse tissues. Knockdown of LAIR2 markedly improved cell viability and inhibited the invasive ability of HTR8/SVneo cells.
References
- Liu, Zhao, Zhou, Huang, Chen, Wang, Hao, Xu, He, Wang
- Human-specific LAIR2 contributes to the high invasiveness of human extravillous trophoblast cells Reprod Biol 19(3) 287-292
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