Biotinylated Human KIR3DL3 Protein
Catalog No: KIR3DL3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human KIR3DL3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln26-Leu322 (UniProt accession: Q8N743).
Product Details
- Molecular Aliases
- CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3
- Protein Length
- Gln26-Leu322
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.
Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human KIR3DL3, His-Avi tag with an affinity constant of 0.254 μM as determined in SPR assay (Biacore T200).
Background
Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.
References
- Jones DC, Hiby SE, Moffett A, Trowsdale J, Young NT. Nature of allelic sequence polymorphism at the KIR3DL3 locus. Immunogenetics. 2006;58(8):614-627. doi:10.1007/s00251-006-0130-5
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