Biotinylated Human Kallikrein 7/KLK7 Protein (pro form)
Catalog No: KLK7-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. The protein needs to be activated by Thermolysin for an activated form. It contains amino acid residues Glu23-Arg253 (UniProt accession: P49862-1).
Product Details
- Molecular Aliases
- Kallikrein-7; hK7; Serine protease 6; hSCCE; KLK7; PRSS6; SCCE
- Protein Length
- Glu23-Arg253
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 28.11 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Kallikrein 7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Kallikrein 7 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Kallikrein 7, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Kallikrein 7 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Human Kallikrein 7, His-Avi tag on Streptavidin-Biosensor can bind Anti-Kallikrein 7 Antibody, hFc Tag with an affinity constant of 0.42 nM as determined in BLI assay.
Background
Kallikrein 7 (KLK7) is a secreted serine protease with chymotrypsic protease activity. Abnormally high expression of KLK7 is closely related to the occurrence and development of various types of cancer. Therefore, KLK7 has been identified as a potential target for cancer drug development design in recent years. KLK7 mediates various biological and pathological processes in tumorigenesis, including cell proliferation, migration, invasion, angiogenesis, and cell metabolism, by hydrolyzing a series of substrates such as membrane proteins, extracellular matrix proteins, and cytokines.
References
- Xiang F, Wang Y, Cao C, Li Q, Deng H, Zheng J, Liu X, Tan X. The Role of Kallikrein 7 in Tumorigenesis. Curr Med Chem. 2022;29(15):2617-2631. doi: 10.2174/0929867328666210915104537. PMID: 34525904.
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