Biotinylated Human Kallikrein 2/KLK2 Protein (active form)
Catalog No: KLK2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Kallikrein 2/KLK2 Protein (active form) is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile25-Pro261 (UniProt accession: P20151-1).
Product Details
- Molecular Aliases
- KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2;hGK1
- Protein Length
- Ile25-Pro261
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 29.21 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 85% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 8.0).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Kallikrein 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%.
The purity of Biotinylated Human Kallikrein 2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Kallikrein 2, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 28.0ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Human Kallikrein 2, His-Avi tag on Streptavidin-Biosensor can bind Anti-Kallikrein 2 Antibody, hFc Tag with an affinity constant of 0.24 nM as determined in BLI assay. (QC Test)
Background
Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target.
References
- Jairajpuri DS, Hussain A, Alajmi MF, Mohammad T, Shamsi A, Hassan MI. Structure-based identification of bioactive phytochemicals targeting kallikrein-related peptidase 2 for prostate cancer therapy. Front Chem. 2025 Mar 26;13:1553987. doi: 10.3389/fchem.2025.1553987. PMID: 40207180; PMCID: PMC11979280.
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