Biotinylated Human IL-6 R alpha/CD126 Protein
Catalog No: IL6-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-6 R alpha/CD126 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu20-Pro365 (UniProt accession: P08887-1).
Product Details
- Molecular Aliases
- IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80;IL6R subunit alpha; IL6R-alpha; IL6RA; IL6R1
- Protein Length
- Leu20-Pro365
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-6, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.78μg/ml determined by ELISA. (QC Test)
Background
The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.
References
- Schmidt-Arras D, Rose-John S. IL-6 pathway in the liver: from physiopathology to therapy[J]. Journal of Hepatology, 2016:S0168827816000830.D
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