Biotinylated Human IL-25/IL-17E Protein
Catalog No: IL25-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-25/IL-17E Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Tyr33-Gly177 (UniProt accession: Q9H293-1).
Product Details
- Molecular Aliases
- IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25;IL25;Interleukin 25; IL 25
- Protein Length
- Tyr33-Gly177
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-25 is greater than 95% as determined by SEC-HPLC.
The purity of Biotinylated Human IL-25 is greater than 95% and the molecular weight of this protein is around 40-60 kDa as determined by SEC-MALS.
Immobilized Human IL-17RB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 48.3ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human IL-25, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IL-25 Antibody, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.
Background
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
References
- Xu M, Dong C. IL-25 in allergic inflammation. Immunol Rev. 2017 Jul;278(1):185-191. doi: 10.1111/imr.12558. PMID: 28658555.
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