Biotinylated Human IL-2 R beta/CD122 Protein
Catalog No: IL2-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-2 R beta/CD122 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala27-Asp239 (accession: NP_000869).
Product Details
- Molecular Aliases
- CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6; IL-15 R β; IL-2 R β; IL-2R subunit β;IL15R beta; IL2R beta; IL2R subunit beta; IL15R β; IL2R β; IL2R subunit β;IL-15 R b; IL15R b; IL-2 R b; IL2R b; IL-2R subunit b; IL2R subunit b
- Protein Length
- Ala27-Asp239
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-15Ra&IL-15, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 38.0ng/ml determined by ELISA (QC Test).
Biotinylated Human IL-2 R beta, His-Avi tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 μM as determined in SPR assay (Biacore T200).
Biotinylated Human IL-2 R beta, His-Avi tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).
Background
IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
References
- Smith K A. The structure of IL2 bound to the three chains of the IL2 receptor and how signaling occurs[J]. Medical Immunology, 2006, 5(1).
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