Biotinylated Human IL-2 Protein
Catalog No: IL2-HB006
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala21-Thr153 (UniProt accession: P60568).
Product Details
- Molecular Aliases
- IL2; T-cell growth factor; TCGF; Aldesleukin;Interleukin-2; Interleukin 2
- Protein Length
- Ala21-Thr153
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.32 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-2 R alpha, His Tag (Cat. CD5-HM125) at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 93.3ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Human IL-2, His-Avi tag on Streptavidin-Biosensor can bind Human IL-2 R alpha, His Tag (Cat. CD5-HM125) with an affinity constant of 35.70 nM as determined in BLI assay.
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
References
- Sim G C, Martin-Orozco N, Jin L, et al. IL-2 therapy promotes suppressive ICOS Treg expansion in melanoma patients[J]. The Journal of clinical investigation, 2013, 124(1).
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