Biotinylated Human IL-18BPa Protein
Catalog No: IL18BPA-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-18BPa Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr31-Gly194 (UniProt accession: O95998-2).
Product Details
- Molecular Aliases
- IL-18BP; Igifbp; IL-18BPA;IL18BP; IL18BPA
- Protein Length
- Thr31-Gly194
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-18BPa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human IL-18BPa, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag (Cat. IL1-HE018) with an affinity constant of 0.90 nM as determined in SPR assay (Biacore T200). (QC Test)
Background
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
References
- Zhou T, Damsky W, Weizman OE, McGeary MK, Hartmann KP, Rosen CE, Fischer S, Jackson R, Flavell RA, Wang J, Sanmamed MF, Bosenberg MW, Ring AM. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature. 2020 Jul;583(7817):609-614. doi: 10.1038/s41586-020-2422-6. Epub 2020 Jun 24. PMID: 32581358; PMCID: PMC7381364.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.