Biotinylated Human IL-17F Protein
Catalog No: IL17F-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-17F Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Arg31-Gln163 (UniProt accession: Q96PD4).
Product Details
- Molecular Aliases
- Cytokine ML-1; IL17F; IL-17F; CANDF6; IL-17F; ML1;Cytokine ML1
- Protein Length
- Arg31-Gln163
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.81 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-17F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17F is greater than 95% as determined by SEC-HPLC.
The purity of Biotinylated Human IL-17F is greater than 95% and the molecular weight of this protein is around 35-50 kDa as determined by SEC-MALS.
Immobilized Biotinylated Human IL-17F, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 86.4ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human IL-17F, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-17Rc, hFc Tag (Cat. IL1-HM2RC) with the EC50 of 8.4ng/ml determined by ELISA.
Background
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
References
- Yang X O, Chang S H, Park H, et al. Regulation of inflammatory responses by IL-17F[J]. Journal of Experimental Medicine, 2008, 205(5):1063-1075.
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