Biotinylated Human IL-13Ra1 Protein
Catalog No: IL13RA1-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-13Ra1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly22-Thr343 (UniProt accession: P78552-1).
Product Details
- Molecular Aliases
- IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074;IL13R alpha 1
- Protein Length
- Gly22-Thr343
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.5 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-13Ra1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated IL-13Ra1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test).
Background
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
References
- Suzuki A, Leland P, Joshi B H, et al. Targeting of IL-4 and IL-13 receptors for cancer therapy[J]. Cytokine, 2015, 75(1):S1043466615002124.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.