Biotinylated Human IL-12 Protein
Catalog No: IL12-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-12 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile23-Ser328 (IL-12B) and Arg23-Ser219 (IL-12A) (accession: P29460(IL-12B) & P29459(IL-12A)).
Product Details
- Molecular Aliases
- CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12; IL12B; IL12p70; IL12 subunit p35; Interleukin-12; IL-12; IL 12
- Protein Length
- Ile23-Ser328
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 34.7 kDa (IL-12B) and 25.5 kDa (IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-12 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-12, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. (QC Test)
Background
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
References
- Lu X. Impact of IL-12 in Cancer. Curr Cancer Drug Targets. 2017;17(8):682-697. doi:10.2174/1568009617666170427102729
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