Biotinylated Human IGF1R/CD221 Protein
Catalog No: IGF1R-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IGF1R/CD221 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu31-Asn932 (UniProt accession: P08069).
Product Details
- Molecular Aliases
- CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216
- Protein Length
- Glu31-Asn932
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 80.4 kDa (alpha subunit) and 25.4 kDa (beta subunit). Due to the furin cleavage site and glycosylation, the protein migrates to 110-120 kDa and 52-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IGF1R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IGF1R is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human IGF1R, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IGF-I, hFc Tag with the EC50 of 1.13μg/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human IGF1R, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Background
The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.
References
- Riedemann J, Macaulay VM. IGF1R signalling and its inhibition. Endocr Relat Cancer. 2006 Dec;13 Suppl 1: S33-43. doi: 10.1677/erc.1.01280. PMID: 17259557.
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