Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein
Catalog No: EBVLMP1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-terminus. It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119 (B2M) and GGDPHLPTL peptide (accession: P13747(HLA-E*01:03) & P61769(B2M) &GGDPHLPTL).
Product Details
- Molecular Aliases
- EBV LMP1; GGDPHLPTL
- Protein Length
- Gly22-Thr302
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is expressed in germinal-center-derived, mononuclear Hodgkin (H) and multinuclear, diagnostic Reed-Sternberg (RS) cells in classical EBV-positive Hodgkin's lymphoma (cHL). LMP1 expression in EBV-negative H-cell lines results in a significantly increased number of RS cells.
References
- Knecht H, Mai S. LMP1 and Dynamic Progressive Telomere Dysfunction: A Major Culprit in EBV-Associated Hodgkin's Lymphoma. Viruses. 2017 Jun 27;9(7):164. doi: 10.3390/v9070164. PMID: 28654015; PMCID: PMC5537656.
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