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Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer Protein

Catalog No: HLA-HB002

Species
Human
Expression System
E.coli
Tag
His, Avi

Product overview

Recombinant Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer Protein is expressed in E. coli with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly25-Val309(HLA-B*27:05), Ile21-Met119(B2M) and LRVMMLAPF peptide (accession: AAA36221.1(HLA-B*27:05) & P61769(B2M) &LRVMMLAPF).

Product Details

Molecular Aliases
yeiH; b2158; JW2145;
Protein Length
Gly25-Val309
Expression System
E.coli
Theoretical Molecular Weight
The protein has a predicted MW of 36.08 kDa (HLA-B*27:05) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
State
Lyophilized
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution Advice
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data Display

Bis-Tris PAGE
HLA-HB002 Bis-Tris-PAGE-white result

Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC
HLA-HB002 SEC-HPLC result

The purity of Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer is greater than 95% as determined by SEC-HPLC.

Background

YeiH is a conserved bacterial inner membrane protein widely distributed in gut microflora like Escherichia coli. Its immunogenic segment LRVMMLAPF (YeiH232-240) is an HLA-B*27-bound microbial epitope. By mimicking self-antigens, this bacterial peptide serves as an environmental trigger that activates cross-reactive, pathogenic CD8+ T cells, driving the immune response in conditions such as ankylosing spondylitis (AS) and acute anterior uveitis (AAU).

References

  1. Mechanistic Insights from Human Studies in Axial Spondyloarthritis: A T Cell Story. Curr Rheumatol Rep. 2026 Mar 5;28(1):6. doi: 10.1007/s11926-026-01213-3. PMID: 41784664; PMCID: PMC12963204.
  2. Mucosal signatures of pathogenic T cells in HLA-B*27+ anterior uveitis and axial spondyloarthritis. JCI Insight. 2024 Jul 18;9(16):e174776. doi: 10.1172/jci.insight.174776. PMID: 39024572; PMCID: PMC11343591.
  3. Autoimmunity-associated T cell receptors recognize HLA-B*27-bound peptides. Nature. 2022 Dec;612(7941):771-777. doi: 10.1038/s41586-022-05501-7. Epub 2022 Dec 7. PMID: 36477533; PMCID: PMC10511244.

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