Biotinylated Human HLA-A*25:01&B2M&NUTM1 (DVYENFRQW) Monomer Protein
Catalog No: HLA-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*25:01&B2M&NUTM1 (DVYENFRQW) Monomer Protein is expressed in E. coli with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly25-Pro307(HLA-A*25:01), Ile21-Met119(B2M) and DVYENFRQW peptide (accession: CDK41178.1(HLA-A*25:01) & P61769(B2M) &DVYENFRQW).
Product Details
- Molecular Aliases
- NUTM1; C15orf55; NUT; FAM22H
- Protein Length
- Gly25-Pro307
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 36.1 kDa (HLA-A*25:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*25:01&B2M&NUTM1 (DVYENFRQW) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*25:01&B2M&NUTM1 (DVYENFRQW) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Nuclear protein of testis (NUT), a protein product of the NUTM1 gene (located on the long arm of chromosome 15) with highly restricted physiologic expression in post-meiotic spermatids, is the oncogenic driver of a group of emerging neoplasms when fused with genes involved in transcription regulation.
References
- Luo W, Stevens TM, Stafford P, Miettinen M, Gatalica Z, Vranic S. NUTM1-Rearranged Neoplasms-A Heterogeneous Group of Primitive Tumors with Expanding Spectrum of Histology and Molecular Alterations-An Updated Review. Curr Oncol. 2021 Nov 7;28(6):4485-4503. doi: 10.3390/curroncol28060381. PMID: 34898574; PMCID: PMC8628659.
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