Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein
Catalog No: HLAA0301-HB001
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and FTWTPSSGK peptide (accession: NP_002107.3(HLA-A*03:01) & P61769(B2M) &FTWTPSSGK).
Product Details
- Molecular Aliases
- MHC; IGA2
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.26 kDa (HLA-A*03:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human HLA*03:01&B2M&IGA2 (FTWTPSSGK) Monomer, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0 ng/ml determined by ELISA.
Background
The immunoglobulin variable region is a part of an antibody molecule responsible for antigen recognition and binding. It is located at the N-terminal end of each antibody chain and is highly diverse due to the presence of variable (V), diversity (D), and joining (J) gene segments in B cells that undergo somatic recombination during antibody production. This variability allows the immune system to recognize a wide range of antigens.
References
- [1] Kang TH, Jung ST. Reprogramming the Constant Region of Immunoglobulin G Subclasses for Enhanced Therapeutic Potency against Cancer. Biomolecules. 2020 Mar 1;10(3):382. doi: 10.3390/biom10030382.
- [2] Cory S. Immunoglobulin variable region genes. Surv Synth Pathol Res. 1984;3(2):149-64. doi: 10.1159/000156923.
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