Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein
Catalog No: MAGEA1-HB002
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KVLEYVIKV).
Product Details
- Molecular Aliases
- MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.
Background
MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.
References
- Wang D, Wang J, Ding N, Li Y, Yang Y, Fang X, Zhao H. MAGE-A1 promotes melanoma proliferation and migration through C-JUN activation. Biochem Biophys Res Commun. 2016 May 13;473(4):959-965. doi: 10.1016/j.bbrc.2016.03.161. Epub 2016 Apr 1. PMID: 27045082.
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