Biotinylated Human HLA-A*02:01&B2M&EBV BMLF1 (GLCTLVAML) Monomer Protein
Catalog No: HLAA0201-HB013
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&EBV BMLF1 (GLCTLVAML) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GLCTLVAML peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &GLCTLVAML).
Product Details
- Molecular Aliases
- Mta; ORF57; EB2; SM; BMLF1; BSLF2
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.33 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-A*02:01&B2M&EBV BMLF1 (GLCTLVAML) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&EBV BMLF1 (GLCTLVAML) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Epstein-Barr virus (EBV) encodes a gene product in the BamHI-M leftward reading frame 1 (BMLF1) that functions as a promiscuous transactivator acting upon many other enhancer-promoter combinations. It also promotes the nuclear export of a subset of early and late viral mRNAs and is essential for the production of infectious virions.
References
- (1) Wong KM, Levine AJ. Characterization of proteins encoded by the Epstein-Barr virus transactivator gene BMLF1. Virology. 1989 Jan;168(1):101-11. doi: 10.1016/0042-6822(89)90408-x. PMID: 2535897. (2) Medina-Palazon C, Gruffat H, Mure F, Filhol O, Vingtdeux-Didier V, Drobecq H, Cochet C, Sergeant N, Sergeant A, Manet E. Protein kinase CK2 phosphorylation of EB2 regulates its function in the production of Epstein-Barr virus infectious viral particles. J Virol. 2007 Nov;81(21):11850-60. doi: 10.1128/JVI.01421-07. Epub 2007 Aug 15. PMID: 17699575; PMCID: PMC2168784.
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