Biotinylated Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein
Catalog No: HLAA0201-HB003
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &AAPGAPAAV).
Product Details
- Molecular Aliases
- AAPGAPAAV; ARF
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Background
Auxin Response Factors (ARFs) are a family of plant-specific transcription factors that play crucial roles in regulating plant growth and development. ARFs bind to auxin response elements (AuxREs) in the promoters of auxin-responsive genes, where they activate or repress transcription. They also recruit a second family of transcription factors, the Aux/IAA repressors, which confer an auxin response to the genes.
References
- [1] Jing H, Korasick DA, Emenecker RJ, Morffy N, Wilkinson EG, Powers SK, Strader LC. Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning. Nat Commun. 2022 Jul 11;13(1):4015. doi: 10.1038/s41467-022-31628-2.
- [2] Li SB, Xie ZZ, Hu CG, Zhang JZ. A Review of Auxin Response Factors (ARFs) in Plants. Front Plant Sci. 2016 Feb 3;7:47. doi: 10.3389/fpls.2016.00047.
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