Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling)
Catalog No: FIBRONECTIN-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln32-Thr345 (UniProt accession: P02751-1).
Product Details
- Molecular Aliases
- FN; CIG; FN1; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; SMDCF
- Protein Length
- Gln32-Thr345
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.93 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Fibronectin (32-345) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fibronectin (32-345) is greater than 90% as determined by SEC-HPLC.
Background
Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.
References
- Ruoslahti E. Fibronectin. J Oral Pathol. 1981 Feb;10(1):3-13. doi: 10.1111/j.1600-0714.1981.tb01242.x. PMID: 6782207.
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