Biotinylated Human FGL2 Protein
Catalog No: FGL2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His-Avi, Flag
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FGL2 Protein is expressed in HEK293 cells with a His-Avi tag and Flag tag at the N-terminus. It contains amino acid residues Val205-Pro439 (UniProt accession: Q14314).
Product Details
- Molecular Aliases
- Fibroleukin; pT49; FGL2; fibrinogen-like 2;
- Protein Length
- Val205-Pro439
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGL2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human FGL2, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
Background
Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.
References
- Feng Y, Guo C, Wang H, Zhao L, Wang W, Wang T, Feng Y, Yuan K, Huang G. Fibrinogen-Like Protein 2 (FGL2) is a Novel Biomarker for Clinical Prediction of Human Breast Cancer. Med Sci Monit. 2020 Jul 27;26:e923531. doi: 10.12659/MSM.923531. PMID: 32716910; PMCID: PMC7409386.
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